Journal: Anatomy & Cell Biology

Article Title: Aptamin C enhances anti-cancer activity NK cells through the activation of STAT3: a comparative study with vitamin C

doi: 10.5115/acb.24.120

Figure Lengend Snippet: Comparison of vitamin C levels in the tissues of Gulo knockout (KO) mice treated with vitamin C and Aptamin C. Vitamin C levels were measured in the stomach, spleen, brain, liver, lung, and adrenal gland of Gulo KO mice (n=5) supplemented with vitamin C (3.3 g/L) and Aptamin C (vitamin C 3.3 g/L, aptamer 33 mg/L) for 2 and 4 weeks. Vitamin C level was measured by using a colorimetric microtiter plate assay kit as described in Materials and Methods. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test and presented as the mean±SD. Three independent experiments were performed. ns, not significant; WT, wild type. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: The homogenates were centrifuged at 14,000 rpm for 30 minutes at 4°C, and the supernatants were used for measuring vitamin C. The tissue homogenates were diluted in PBS, and vitamin C (ascorbic acid) was converted into its oxidized form, dehydroascorbic acid (DHA), after which the total DHA concentration was measured using a colorimetric microtiter plate assay kit (Immundiagnostik AG) according to the manufacturer’s instructions.

Techniques: Comparison, Knock-Out